PFKFB1 anticorps (AA 12-186)

N° du produit ABIN7599810

L’anticorps Lapin Polyclonal anti-PFKFB1 a été validé pour WB, ELISA, IHC et FACS. Il convient pour détecter PFKFB1 dans des échantillons de Humain.

Aperçu rapide pour PFKFB1 anticorps (AA 12-186) (ABIN7599810)

Antigène: PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PFKFB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-PFKFB1 anticorps

Épitope: AA 12-186

Fonction: Anti-PFKFB1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PFKFB1 Antibody Picoband® (ABIN7599810). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PFKFB1 recombinant protein (Position: R12-R186).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Algaier, J., Uyeda, K. Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6,2-kinase:fructose-2,6-bisphosphatase. Biochem. Biophys. Res. Commun. 153: 328-333, 1988. 2. Batra, R. S., Hatchwell, E., Rider, S., Brown, R., Brown, G. K., Craig, I. W. Localization of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB1) within a YAC contig in Xp11.21. Genomics 40: 358-361, 1997. 3. Darville, M. I., Crepin, K. M., Vanderkerckhove, J., Van Damme, J., Octave, J. N., Rider, M. H., Marchand, M. J., Hue, L., Rousseau, G. G. Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone. FEBS Lett. 224: 317-321, 1987.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur PFKFB1

Antigène: PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))

Autre désignation: PFKFB1

Sujet:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 2,ADAM 2,Cancer/testis antigen 15,CT15,Fertilin subunit beta,PH-30,PH30,PH30-beta,ADAM2,FTNB,

Tissue Specificity: Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.

Background: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene.

Poids moléculaire: 55 kDa

ID gène: 5207

UniProt: P16118

Pathways: Regulation of Carbohydrate Metabolic Process